Artificial Sea Water (from Methods in Cell Biol. Vol. 74)

Per Liter:

28.3g NaCl

0.77g KCl

5.41g MgCl2•6H2O

3.42g MgSO4 or 7.13g MgSO4•7H2O

0.2g NaHCO3

1.56g CaCl2 dihydrate (add last)

adjust pH to 8.2, salinity should be in the range of 34-36 ppt