Techniques that everyone in our lab needs to master:

1. Molecular Biology skill set
-PCR
-Plasmid mini prep
-DNA/RNA gel electrophoresis
-Ligation, transformation

2. Sea Urchin skill set
-Micro-injection (?500 embryos/10 min, preferably ?1000 embryos/10min)
-Embryo culture
-Spawning
-DNA/RNA extraction from embryos

 

Optional skills:
-QPCR
-Programming